FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l01_n01_N_Light015.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l01_n01_N_Light015.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6677165
Sequences flagged as poor quality0
Sequence length101
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG128050.090.807035
GTATAGG134450.089.133486
CTATACG57000.088.664786
GTATACG63550.088.570116
GCTATAC65100.088.504925
GTATAGC73450.087.625786
CGTATAC54500.087.503385
CGTATAG74550.087.4803545
TATACGG78800.087.342687
CCTATAG50750.087.230325
TATAGGC81400.086.361797
TATAGCG83950.086.11497
GTATACC52200.085.989556
TTATACG84600.084.947956
GCTATAG91900.084.8682565
CCTATAC50950.084.557225
TATACTG115350.084.497557
TTATAGC106900.084.200736
GGTATAC73850.083.9358755
AGTATAG99150.083.692745
Produced by FastQC (version 0.11.5)