FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l01_n01_N_Light014.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l01_n01_N_Light014.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9393994
Sequences flagged as poor quality0
Sequence length101
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG154850.089.998255
GTATAGG169150.089.8874056
CGTATAG97150.089.179955
CGTATAC73000.089.1421055
GTATAGC94200.088.090126
TATACGG98950.088.037717
CCTATAG63550.087.74815
GTATACG82450.087.624136
CTATACG69550.087.144256
GCTATAC87050.086.7588045
TATAGCG107200.086.18077
TATAGGC110600.085.93647
GGTATAC93250.084.3013155
TTATACG108750.083.947796
CCTATAC64000.083.939785
TATACTG159700.083.9338767
GTATACC67600.083.7557756
GCTATAG116150.083.547675
GATATAG138700.083.32035
TTATAGC134650.082.792686
Produced by FastQC (version 0.11.5)