FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l01_n01_N_Light013.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l01_n01_N_Light013.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9815775
Sequences flagged as poor quality0
Sequence length101
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG179700.090.187855
GTATAGG188850.089.917916
CGTATAG107000.089.360825
CTATACG80550.088.453066
GCTATAC95900.088.212975
CGTATAC82250.088.18415
GTATACG94950.088.045036
TATACGG113200.088.033147
GTATACC72150.087.559336
CCTATAG69250.087.17925
CCTATAC70350.086.9638755
GTATAGC112150.086.824396
TATAGGC118500.085.5788967
GCTATAG128350.085.154565
CTATAGG119300.084.766136
TTATACG122450.084.5638666
TATAGGT218200.084.092477
TATAAGG171350.084.0212257
GTTATAG216550.083.811965
GTATAGT216650.083.79526
Produced by FastQC (version 0.11.5)