FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l01_n01_N_Light012.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l01_n01_N_Light012.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5022541
Sequences flagged as poor quality0
Sequence length101
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG93550.090.3278665
GTATAGG97200.089.8686
CGTATAG53500.089.583285
CCTATAC34000.087.734485
GCTATAC49350.087.5878455
CGTATAC42100.087.439815
GTATACG48950.087.4302446
CTATACG38650.086.642156
TATAGGC64100.086.551467
CCTATAG38550.086.374045
ATACGCG9850.086.318148
TATACGG58500.086.311177
GGTATAC56050.086.1856465
GTATAGC55250.086.1440056
TATAGCG61950.085.951597
GTATACC38950.085.852866
TTATACG61400.085.019586
CTATAGG68050.084.738386
AGTATAC43250.084.126395
GTATAGT106000.083.706776
Produced by FastQC (version 0.11.5)