FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l01_n01_N_Light011.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l01_n01_N_Light011.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3817523
Sequences flagged as poor quality0
Sequence length101
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG69250.090.472985
GTATAGG73450.089.050426
CGTATAG41100.088.990325
GTATAGC42100.087.7791446
TATACGG42650.087.426777
CTATACG28850.086.438526
GTATACG33250.086.428626
GCTATAC38450.086.3524555
CGTATAC28350.085.6173255
TATAGCG45350.085.5733647
TATAGGC44600.085.521357
TTATACG45950.085.282976
TATACTG67550.084.522627
CCTATAC25250.084.465395
GTATACC27350.084.40596
ATACGGC8500.084.3812948
GCTATAG51050.084.0206155
CCTATAG25850.083.97495
TATAACG33750.083.3185657
GGTATAC44400.083.1250465
Produced by FastQC (version 0.11.5)