FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l01_n01_N_Light010.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l01_n01_N_Light010.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2833239
Sequences flagged as poor quality0
Sequence length101
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG54450.091.420575
CGTATAG32450.090.166785
CCTATAG21900.090.008715
GTATAGG56500.089.7849046
GTATACG26550.089.6300666
GTATAGC30600.088.788186
TATACGG32700.088.6058967
CTATACG22400.087.999586
TATAGGC32300.087.791477
GCGCCTA1950.087.740762
GCTATAC29050.087.639365
CGTATAC23650.087.566085
CCTATAC21600.085.761315
GTATAAG50400.085.572826
GGTATAC31800.085.4376755
GTATACC22400.084.818886
GTATAGT57650.084.7806246
TATAGCG38450.084.7438667
AGTATAG41600.084.606835
TATAACG26000.084.583997
Produced by FastQC (version 0.11.5)