FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l01_n01_N_Light009.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l01_n01_N_Light009.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3361597
Sequences flagged as poor quality0
Sequence length101
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG62850.090.3890845
GTATAGG67600.090.221166
GTATACG33950.088.563356
CGTATAG37950.087.990065
GCTATAC33200.087.845675
CTATACG26950.087.596696
TATAGCG45350.086.82957
CCTATAG24150.086.738435
TTATACG41200.086.6983266
CTATAGG41550.086.4252856
GTATAGC37600.086.4088756
GTATACC25950.086.396216
GCTATAG44500.086.246485
TATACGG39950.086.20087
TATAGGC42050.086.075397
CGTATAC28000.085.8385855
CCTATAC24900.085.461155
TATAGGT80450.084.725857
AGTATAC30800.084.51235
ATACGCG9000.084.4425058
Produced by FastQC (version 0.11.5)