FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l01_n01_N_Light008.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l01_n01_N_Light008.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6510082
Sequences flagged as poor quality0
Sequence length101
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG126550.089.781395
GTATAGG130600.089.033936
CGTATAC56750.088.386385
GTATACG66750.088.167266
GCTATAC64950.088.051085
CCTATAC46950.087.9167255
CTATACG51500.087.5278556
CGTATAG73950.087.483525
TATACGG81450.087.359087
TATAGGC85000.086.225237
GTATAGC72850.085.9356166
CCTATAG48200.085.7352755
GTATACC51200.084.700926
TATACTG114000.084.248787
TTATACG85050.084.219836
GTATAGT137150.084.089196
GCTATAG89300.083.9881445
AGTATAC56650.083.763135
GGTATAC74400.083.762235
GTTATAG148950.083.2952045
Produced by FastQC (version 0.11.5)