FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l01_n01_N_Light007.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l01_n01_N_Light007.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3618211
Sequences flagged as poor quality0
Sequence length101
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG70600.091.094995
CGTATAG44800.088.847775
GTATAGG77600.088.6925666
CGTATAC33500.088.616745
TATACGG48500.088.239627
GTATACG40900.088.029146
GCTATAC38500.087.964895
CTATACG32750.087.745456
GTATAGC42900.087.689676
CCTATAC28650.087.536635
GTATACC28350.086.9550556
CCTATAG27550.086.721535
CTATAGG48150.086.31626
GGTATAC43750.086.203125
TATAGCG49100.086.000477
TATAACG36350.085.58897
TTATACG48800.085.555826
GCTATAG54000.085.057625
TATAGGC52550.085.054537
AGTATAC33750.085.004855
Produced by FastQC (version 0.11.5)