FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l01_n01_N_Light006.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l01_n01_N_Light006.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5326193
Sequences flagged as poor quality0
Sequence length101
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG101750.090.889255
GTATAGG104100.089.476276
CGTATAG59800.088.88125
CGTATAC46100.088.712185
GTATACG53550.088.522176
TATACGG60650.087.713867
GTATAGC58650.087.303526
TATAGGC67200.086.656717
CCTATAC37450.086.626325
CTATACG40700.086.594546
GCTATAC51500.086.512055
CCTATAG37700.085.295945
GTATACC42050.084.944026
CGCGCTA3700.084.773432
GGTATAC63400.084.508585
TATAGCG64700.084.425687
CCGTATA45800.084.315244
TATACTG93900.083.919297
GTTATAG124200.083.75365
ATACGCG10850.083.176538
Produced by FastQC (version 0.11.5)