FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l01_n01_N_Light005.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l01_n01_N_Light005.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3337392
Sequences flagged as poor quality0
Sequence length101
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG61350.089.9659355
GTATAGG67150.089.056636
CGTATAG38450.088.821715
TATACGG40250.088.7439047
CGTATAC29850.088.633415
GTATACG33350.088.161996
CGCGCTA2050.088.096182
CCTATAC26100.087.3549045
GCTATAC34050.087.047045
CTATACG26350.086.8866356
GTATAGC36450.086.65846
CCTATAG24750.085.5945745
GGTATAC37150.084.897685
TATAGGC41900.084.795767
GTATACC27750.084.557196
CTATAGG42150.084.40556
TATAGCG42450.084.368487
GTTATAG76600.084.208825
GCTATAG47050.084.19635
GTATAGT70500.084.151116
Produced by FastQC (version 0.11.5)