FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l01_n01_N_Light004.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l01_n01_N_Light004.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3363573
Sequences flagged as poor quality0
Sequence length101
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG68750.090.57596
CGTATAG39800.090.343215
GGTATAG64700.089.858635
TATACGG42500.089.4095157
CTATACG26300.089.2182856
GCTATAC34900.088.056525
GTATAGC37750.087.82566
CCTATAG27100.087.811455
TATAGGC45250.087.5447547
GTATACG36650.087.221546
CGTATAC30900.087.1585
CGCGCTA2900.086.858512
TATAGCG44900.086.851937
AGTATAC28150.085.885935
GTATACC26150.085.733976
GGTATAC38850.085.583435
TTATACG41800.085.225126
GCTATAG46550.084.895835
CCTATAC26200.084.66395
GATATAG54600.084.2973255
Produced by FastQC (version 0.11.5)