FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l01_n01_N_Light003.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l01_n01_N_Light003.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3491577
Sequences flagged as poor quality0
Sequence length101
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG65250.089.975365
CGTATAG38800.089.244495
GTATAGG71400.088.944456
GTATACG35200.088.5210956
CGTATAC28500.088.4983755
TATACGG40100.087.53587
GTATAGC38750.087.153246
TATAGGC43450.086.7993247
CTATACG29900.086.737536
GTATACC28750.086.572326
CCTATAC23000.086.117995
GCTATAC35600.085.9253855
CCTATAG29600.085.5305255
CTATAGG42400.085.476036
TATAGCG48000.085.10267
TATAGGT79100.084.84997
AGTATAC29250.084.605285
GCGTATA49200.084.281994
GGTATAC42350.084.006725
ATAGGGC12550.083.643888
Produced by FastQC (version 0.11.5)