FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l01_n01_N_Light002.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l01_n01_N_Light002.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3686115
Sequences flagged as poor quality0
Sequence length101
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG66950.090.812695
CTATACG29550.089.5333566
GTATAGG72950.089.529176
CGTATAG43300.089.1845865
GTATAGC41450.088.008356
CCTATAC26250.087.3987055
TATACGG44150.087.252387
CCTATAG28600.087.1927645
GTATACC25650.086.665386
CGTATAC29850.086.405755
TATAGCG49350.086.33617
CTATAGG45750.085.9659356
CCGTATA31800.085.887094
GCTATAC35750.085.8308945
GTATACG38100.085.7736
GGTATAC43100.085.7411965
TATAGGC43900.085.58537
TATAACG35050.085.241247
AGTATAG57550.083.856285
TATACCG35700.083.689247
Produced by FastQC (version 0.11.5)