FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l01_n01_N_Light001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l01_n01_N_Light001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4447340
Sequences flagged as poor quality0
Sequence length101
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG83800.091.1449665
GTATAGG88100.090.470456
CTATACG39750.089.383046
TATACGG54550.089.3394557
CGTATAC38250.089.1627965
GCTATAC49550.088.384875
CGTATAG50800.088.1736155
GTATACG45100.088.153516
CTATAGG51900.088.135256
CCTATAG33700.088.092885
GTATAGC49950.088.0574656
GTATACC34300.087.382796
CCTATAC31350.087.272135
TATAGGC57600.085.845777
GCTATAG56950.085.824715
AGTATAC37700.085.423835
TTATACG54850.085.386856
ATACGGC11800.085.337458
TATACTG78400.085.1237957
GTATAGT95350.084.986316
Produced by FastQC (version 0.11.5)