FastQCFastQC Report
Tue 4 Aug 2020
HKMLKDRXX_l02_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLKDRXX_l02_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17150178
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG994490.5798715325287003No Hit
CTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTC312630.1822896532035994No Hit
CAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAA236510.1379052742193113No Hit
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC203620.11872763069864349No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGACT117100.037.9491962
CTCAGAG200150.035.1750831
CAGACTC145650.032.8891371
TCAGAGA212450.032.791832
CAGAGAG230950.029.2193893
AGAACCC252700.026.9302378
GAACCCA258150.026.277479
AACTGTG65400.025.8271882
CAACTGT67150.025.616591
GTTCACT62750.024.8315938
GAGAGAA288050.024.6350925
TGTGTTC65750.024.3632895
GAGAACC289050.023.2677757
TTCACTA72300.022.3535449
CTGTGTT71400.022.2323464
GACTCAG226100.021.1904563
AGAGAGA356250.020.2241784
GAATCCA107600.020.2091664
AGAATCC107200.019.8788813
ACTCAGA287750.019.8747234
Produced by FastQC (version 0.11.5)