FastQCFastQC Report
Tue 4 Aug 2020
HKMLKDRXX_l02_n02_A2_34___297.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLKDRXX_l02_n02_A2_34___297.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5688318
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTC232740.4091543405273756No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG168990.29708254707279025No Hit
CAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAA144070.2532734632627782No Hit
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC121890.2142812690851672No Hit
AGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGC110480.19422261554294257No Hit
CCAAGACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTT84550.14863796292682652No Hit
AAGAAAGCGAGCTTAGTGATACTTGTGGGCCAGGGCATTAGCCACACCAG70760.1243952957622974No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTTTTTTTTTTTTTGGGG70300.12358662086050744No Hit
CTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTCA69170.12160009338437128No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTTTTTTTTTTTTTTGGG61650.10838001672902253No Hit
GCAGAATCCAGATGCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGT60190.1058133529102979No Hit
CTCAAGGGCACCTTTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCA57010.10022294815444566No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGACT53250.038.8076062
AGACTCA66450.033.2810062
CAGACTC70950.032.011941
GACTCAG72650.030.7361493
AGAACCC118850.030.622388
CTCAGAG129900.030.0533711
GAGAACC121250.029.9564487
CAACTGT43650.029.5907381
TCAGAGA129500.029.0595722
AGAGAAC125850.028.976716
GAACCCA127200.028.9541599
ACTCAGA87100.028.6335144
CAGAGAG148550.024.9392033
TTCACTA50700.024.738459
GTGTTCA53950.024.7263896
CACAACT35000.023.8423961
TGTTCAC54550.023.78997
GTTCACT54450.023.7004438
GGTTTTT346500.023.67267230-34
AACTGTG53650.023.651572
Produced by FastQC (version 0.11.5)