FastQCFastQC Report
Tue 4 Aug 2020
HKMLKDRXX_l02_n02_A2_31___286.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLKDRXX_l02_n02_A2_31___286.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2332605
Sequences flagged as poor quality0
Sequence length151
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG115980.497212344138849No Hit
CTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTC62600.26836948390318976No Hit
CAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAA47010.20153433607490337No Hit
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC41010.17581202132379892No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTTTTTTTTTTTTTGGGG37460.16059298509606212No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTTTTTTTTTTTTTTGGG33590.14400209208159975No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30060.12886879690303332No Hit
AGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGC29290.1255677665099749No Hit
CCAAGACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTT25820.1106916944789195No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCACA5150.043.6771
ACAGACT17250.042.034112
ACGGACT5350.039.29889
AGACTCA24200.031.7600732
CAGACTC25550.031.2392141
GACTCAG25350.031.173363
ACTCAGA29250.029.7433914
CACGGAC7400.029.3916978
AGAACCC36800.029.1574158
GAGAACC38950.027.3618227
GAACCCA39350.027.2679259
AGAGAAC39350.026.7151976
CGCACAC8450.026.6008782
CTCAGAG44350.025.8500751
GGTTTTT228400.024.53048730-34
TCAGAGA43950.024.4170862
ACACGGA9650.023.297
AAGGGGG373050.022.88967365-69
GGGTTTT217550.022.54133630-34
AGGGGGG558750.022.53032165-69
Produced by FastQC (version 0.11.5)