FastQCFastQC Report
Tue 4 Aug 2020
HKMLKDRXX_l02_n02_A2_28___278.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLKDRXX_l02_n02_A2_28___278.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8447625
Sequences flagged as poor quality0
Sequence length151
%GC66

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG438370.518926917328835No Hit
CTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTC319220.37788135718619137No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTTTTTTTTTTTTTTGGG244840.28983294121128717No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTTTTTTTTTTTTTGGGG207220.24529971441677395No Hit
CAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAA203920.24139329101374646No Hit
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC188200.22278451043932468No Hit
AGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGC134890.15967801601041715No Hit
CCAAGACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTT119790.14180316952989747No Hit
CTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTCA92450.1094390435181486No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCACA14200.067.957391
ACAGACT49300.065.0055542
ACGGACT15550.062.47539
ACACACG21050.046.151595
CACGGAC21200.045.483078
AGACTCA72550.044.6687774
ACACGGA21850.044.1300247
CAGACTC73650.044.1000633
CGCACAC22050.043.405072
CTCAGAG135850.041.9261
GACTCAG78000.041.826535
TCAGAGA140350.040.5022132
CACACGG24050.040.3946346
ACTCAGA94300.039.2864886
CAACTGT31800.039.244131
AGAACCC143650.039.0633358
GAGAACC147700.037.8449447
AGAGAAC150450.037.1531946
GAACCCA154850.036.4252329
CAGAGAG157450.035.8697853