FastQCFastQC Report
Tue 4 Aug 2020
HKMLKDRXX_l02_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLKDRXX_l02_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17150178
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTC409650.23886049462577005No Hit
CAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAA295970.1725754683129236No Hit
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC262450.1530304816661378No Hit
AGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGC208420.12152643546906626No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC1339950.092.305258
TCGGAAG1426000.084.134133
CGGAAGA1456250.083.988314
GAGCACA1480400.083.733939
ATCGGAA1467250.083.1162642
GATCGGA1437450.082.929041
AAGAGCA1541200.081.601087
GAAGAGC1635700.075.230286
GGAAGAG1645200.074.337925
ACAGACT104750.046.3692972
CAGACTC136650.038.420291
CTCAGAG192400.038.11961
TCAGAGA203950.036.417522
CGGAATA30300.036.3417744
CGGAACA42850.036.1799624
CAGAGAG212350.034.6273273
GAGAGAA219800.033.915085
TGTGTTC54850.033.8118175
TATCGGA26450.033.7684751
AACTGTG58400.031.2046472
Produced by FastQC (version 0.11.5)