FastQCFastQC Report
Tue 4 Aug 2020
HKMLKDRXX_l02_n01_A2_34___297.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLKDRXX_l02_n01_A2_34___297.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5688318
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT63545811.171281211774728TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCGCGTAT1430762.5152602227934513TruSeq Adapter, Index 19 (97% over 40bp)
CTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTC260320.4576396748564338No Hit
CAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAA149740.2632412604217978No Hit
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC130400.22924175476828124No Hit
AGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGC117680.20688013574487218No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCAACACAAAATAACTACAAT116710.20517488649544557TruSeq Adapter, Index 7 (97% over 35bp)
CCAAGACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTT86230.15159138430727676No Hit
CTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTCA80800.1420455044883215No Hit
AAGAAAGCGAGCTTAGTGATACTTGTGGGCCAGGGCATTAGCCACACCAG72880.1281222322661989No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGGAT72210.12694437969185268TruSeq Adapter, Index 19 (97% over 40bp)
CTCAAGGGCACCTTTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCA66890.11759187865376022No Hit
GCAGAATCCAGATGCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGT63390.11143891744448886No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCGCGGAT57970.10191061751470293TruSeq Adapter, Index 19 (97% over 40bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA1505450.089.6663369
AGAGCAC1515450.089.012468
AAGAGCA1549350.087.19127
CGGAAGA1558150.086.460714
TCGGAAG1553250.086.145353
ATCGGAA1564350.085.6958542
GAAGAGC1581500.085.216256
GATCGGA1570950.085.114591
GGAAGAG1623250.083.006625
GAACGGA19100.044.07651
ACAGACT54150.041.1407972
AGAACCC119500.035.916438
GAGAACC123300.034.6919177
CTCAGAG131400.034.2435531
GAACCCA129300.033.530669
TCAGAGA129900.033.5177542
AGACTCA68750.033.3234524
AGAGAAC131200.032.9342656
CAGACTC69300.032.150491
CAACTGT45750.032.0437051
Produced by FastQC (version 0.11.5)