FastQCFastQC Report
Tue 4 Aug 2020
HKMLKDRXX_l02_n01_A2_31___286.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLKDRXX_l02_n01_A2_31___286.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2332605
Sequences flagged as poor quality0
Sequence length151
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCGCATCTCGTAT44126918.917433513175183TruSeq Adapter, Index 16 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCGCATCGCGTAT1133494.859331091204898TruSeq Adapter, Index 16 (97% over 39bp)
CTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTC66690.28590352845852596No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCAACACTCATAAACTACAAT48560.20817926738560538TruSeq Adapter, Index 7 (97% over 35bp)
CAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAA46180.19797608253433394No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45300.1942034763708386No Hit
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC42340.18151380109362708No Hit
AGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGC30910.1325127914927731No Hit
CCAAGACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTT24180.10366092844695093No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCGCATCTCGGAT23400.10031702752930735TruSeq Adapter, Index 16 (97% over 39bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA954650.094.674449
AGAGCAC963300.093.7415168
AAGAGCA978550.092.2509847
CGGAAGA989700.091.087164
GAAGAGC993550.090.748796
TCGGAAG988600.090.660463
ATCGGAA995300.090.151262
GATCGGA998200.089.8209461
GGAAGAG1014600.088.873155
ATTCCGC4250.051.1778647
GAACGGA8400.050.111371
TCGCACA4500.049.9961741
CGCACAT5100.042.6857342
CATTCCG5550.040.49666
GCGGTAT1451.9123036E-840.0414281
AACGGAA13600.033.6150172
ACAGACT19500.032.375492
AGACTCA23700.030.9246442
CAGACTC24350.029.8049681
CGGAATA3650.029.795334
Produced by FastQC (version 0.11.5)