FastQCFastQC Report
Tue 4 Aug 2020
HKMLKDRXX_l02_n01_A2_28___278.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLKDRXX_l02_n01_A2_28___278.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8447625
Sequences flagged as poor quality0
Sequence length151
%GC66

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC340562540.31458546041047TruSeq Adapter, Index 12 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCGCGTATGC3836634.541667036593125TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGGATGC495280.5862949645610452TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATTC483590.5724567555969873TruSeq Adapter, Index 12 (98% over 50bp)
CTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTC352590.4173835841434723No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCAACAATAAATCCCTAAACC225380.2667968807801009TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTTTGC223220.26423994909811926TruSeq Adapter, Index 12 (98% over 50bp)
CAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAA208110.24635326497092377No Hit
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC206490.24443556620943754No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTAAGC175210.2074074074074074TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGG173180.2050043651322117TruSeq Adapter, Index 12 (97% over 49bp)
AGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGC149290.17672422722362793No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCGCGGATGC134140.15879019250972906TruSeq Adapter, Index 12 (97% over 45bp)
CCAAGACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTT123620.1463369882067445No Hit
CTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTCA113200.13400216037051832No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTGTAATCTCGTATGC109440.1295512052204022TruSeq Adapter, Index 12 (98% over 50bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96240.11392551160829226No Hit
CAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTC86540.10244299433272665No Hit
GATCGGAAGAGCACACGTCTGAACTCAGTCACCTTGTAATCTCGTATGCC85280.10095145085157071Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA5208350.0124.657659
AGAGCAC5223550.0124.382368
AAGAGCA5254500.0123.747697
GATCGGA5253650.0123.199141
CGGAAGA5285450.0123.017574
ATCGGAA5277250.0122.7972952
TCGGAAG5269800.0122.796823
GAAGAGC5302400.0122.629796
GGAAGAG5345000.0121.684995
GAACGGA31350.095.148241
ATTCCGC11050.079.39027
TCGCACA12450.076.3657151
AACGGAA44850.070.545462
CGCACAT13000.069.218692
CATTCCG13550.065.2776266
ACGGAAG47850.064.3948063
ACAGACT45700.063.199252
TCGGAAA12750.058.5694163
ATTCGGA8450.057.5391122
GATCGGG20750.056.312431
Produced by FastQC (version 0.11.5)