FastQCFastQC Report
Tue 4 Aug 2020
HKMLKDRXX_l01_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLKDRXX_l01_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16418019
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG503520.30668742678395No Hit
CTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTC256790.15640742040802852No Hit
CAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAA204640.12464353951594281No Hit
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC177600.10817383022884794No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGACT101600.036.0187872
CAGACTC125050.035.1013531
CTCAGAG169300.029.1409761
TCAGAGA181650.027.0873052
CAGAGAG198100.024.5793973
AGAACCC220600.022.7661518
GAACCCA225300.022.3233919
GAGAGAA231550.021.9361135
TGTGTTC49550.021.2036555
GGGTTTT167250.021.0104830-34
CAACTGT52450.019.9190751
AACTGTG52000.019.7886642
GTTCACT52900.019.5903538
GACTCAG219900.019.2760073
GAATCCA97200.019.0090474
AGAATCC96250.018.5191383
GAGAACC270200.018.130017
ACTCAGA277100.018.1210384
CTGTGTT57350.017.6880954
AAGGGGG328400.017.39708565-69
Produced by FastQC (version 0.11.5)