FastQCFastQC Report
Tue 4 Aug 2020
HKMLKDRXX_l01_n02_A1_74___262.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLKDRXX_l01_n02_A1_74___262.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13312206
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTC397140.2983277151810902No Hit
CAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAA357360.2684453651032744No Hit
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC326600.24533875151871898No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTTTTTTTTTTTGGG225450.1693558528165805No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG198440.1490662028517287No Hit
AGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGC197930.14868309579944902No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTTTTTTTTTTGGGG193530.14537785848566348No Hit
CCAAGACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTT159060.11948432889334795No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGACT147600.037.135592
AGACTCA175450.034.6707652
CAGACTC201200.030.905381
ACTCAGA221150.030.6187214
GACTCAG202700.030.115183
AGAACCC274000.023.1253348
GGTTTTT558650.022.11092630-34
CACAGAC263100.021.9519671
AGAGAAC289300.021.827146
GAACCCA296450.021.7653529
GGGTTTT546000.021.45207630-34
GAGAACC297350.021.2362277
AAGGGGG844750.021.22406665-69
TCAGAGA311800.020.9974482
CTCAGAG328150.020.2758081
AGGGGGG1099450.020.03031365-69
GGGAAAA856600.018.94299355-59
AAAAAGG876350.018.9017260-64
GGGGGAA915650.018.86306650-54
GGAAAAA937750.018.63965655-59
Produced by FastQC (version 0.11.5)