FastQCFastQC Report
Tue 4 Aug 2020
HKMLKDRXX_l01_n02_A1_6___148.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLKDRXX_l01_n02_A1_6___148.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27961691
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTC1103820.39476153284148663No Hit
CAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAA738210.26400763816465894No Hit
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC635410.2272430519313013No Hit
GCAGAATCCAGATGCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGT558910.19988419155336493No Hit
AGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGC541110.19351833907326993No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG497200.17781471084849623No Hit
CCAAGACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTT423420.1514286099506643No Hit
GTCAACTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCA373760.13366859679552284No Hit
AAGAAAGCGAGCTTAGTGATACTTGTGGGCCAGGGCATTAGCCACACCAG351510.1257112811954041No Hit
CTCAAGGGCACCTTTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCA305430.10923159117951772No Hit
GCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGG295270.10559804841559833No Hit
CTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTCA291580.10427838573854493No Hit
CGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGGCGCACGTGGACG283410.10135653097661369No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGACT277000.037.614472
AGACTCA336750.034.5577432
CAGACTC359050.032.8415571
GACTCAG372700.031.5515353
ACTCAGA441600.030.5360554
CAACTGT238700.029.9743521
AGAACCC590100.029.0559168
GAGAACC604500.028.1718777
AGAGAAC621950.027.5563096
CTCAGAG652400.027.395281
GAATCCA383550.026.9164054
GAACCCA640750.026.9061959
CACAACT189100.026.8615931
AGAATCC373000.026.6864473
TCAGAGA670700.026.096782
GCAGAAT400150.025.6964021
AACTGTG284950.024.9110492
AATCCAG426650.024.8260445
CAGAGAG703650.024.6040533
ACAACTG200400.023.8794422
Produced by FastQC (version 0.11.5)