FastQCFastQC Report
Tue 4 Aug 2020
HKMLKDRXX_l01_n02_A1_5___146.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLKDRXX_l01_n02_A1_5___146.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23823145
Sequences flagged as poor quality0
Sequence length151
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTTTTTTTTTTTGGG1983230.8324803463186746No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTTTTTTTTTTGGGG1604920.6736809938402339No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1251260.525228721900488No Hit
CTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTC1065830.44739265113821036No Hit
CAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAA802220.3367397545538173No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTTTTTTTTTTTTGG797980.3349599727491899No Hit
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC746250.3132457952130166No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTTTTTTTTTTTTTTGGG577630.24246588769031124No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTTTTTTTTTTTTGGG574460.24113524893543656No Hit
AGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGC483540.20297068250224726No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTTTTTTTTTTTTTGGGG472130.19818122250441747No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTTTTTTTTTTTGGGG459510.19288385307649347No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTTTTTTTTTGTGGG459120.1927201467312565No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTTTTTTTTTTGTGG448410.18822451863513404No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTTTTTTTGTTTGGG433310.18188614475544684No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTTTTTTTTGTGGGG419780.17620679385530333No Hit
CCAAGACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTT418500.17566950123503847No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTTTTTTTTGTTGGG416600.1748719575018328No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTTTTTTTGTTGGGG389320.16342090853243768No Hit
GTCAACTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCA373540.1567970979482348No Hit
GCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGG320050.13434414305919726No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTTTTTTTTTGGGGG312990.13138063845054881No Hit
CTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTCA288440.12107553389781239No Hit
CGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGGCGCACGTGGACG281410.11812462208495142No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTTTTTTGTTTGGGG268970.1129028094317522No Hit
GCAGAATCCAGATGCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGT243920.1023878249492248No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGACT187050.066.9438552
AGACTCA270350.046.4732174
CAGACTC282200.044.598813
GACTCAG295900.042.7054185
ACTCAGA347750.040.882836
CTCAGAG491200.034.3625141
TCAGAGA503800.032.9430472
AGAGAAC507700.032.5580376
AGAACCC506550.032.4458928
GAGAACC509100.032.39737
CACAGAC411950.031.7074721
GAACCCA534350.030.9342469
CAGAGAG544600.030.3520283
CAACTGT109100.030.2635771
CACAACT86600.029.914651
TCGCACA32150.029.7938421
ACGGACT30750.029.7068069
ACAACTG89400.028.4672622
GGTTTTT3806550.026.96014430-34
GGGTTTT3746150.025.88220430-34
Produced by FastQC (version 0.11.5)