FastQCFastQC Report
Tue 4 Aug 2020
HKMLKDRXX_l01_n02_A1_4___135.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLKDRXX_l01_n02_A1_4___135.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20292921
Sequences flagged as poor quality0
Sequence length151
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTTTTTTTTTTTGGG1132010.5578349218429421No Hit
CTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTC1131740.5577018705192811No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTTTTTTTTTTGGGG980990.4834148814751706No Hit
CAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAA805730.3970497889387141No Hit
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC711410.35057052653977216No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG697620.3437750533794519No Hit
AGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGC544000.26807377804309196No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTTTTTTTTTTTTGG438080.21587823655352523No Hit
CCAAGACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTT430030.21191133597770373No Hit
GTCAACTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCA412560.2033024225541508No Hit
GCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGG342330.1686942949218597No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTTTTTTTTTTTTTTGGG329870.16255422272624034No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTTTTTTTTTTTTGGG321680.1585183325751872No Hit
CTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTCA309950.152737991736133No Hit
CGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGGCGCACGTGGACG301730.14868731810467303No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTTTTTTTTTTTTTGGGG298380.14703649612591504No Hit
CGGCTACCGAGGCTCCAGCTTAACGGTATTTGGAGGTCAGCACGGTGCTC282390.13915690106909695No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTTTTTTTTTTTGGGG275840.13592917451361486No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTTTTTTTTTGTGGG269220.13266695317051694No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTTTTTTTTTTGTGG267800.13196720176459564No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTTTTTTTTGTGGGG266580.13136600689471958No Hit
GTCAAGGCCGCCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGC245500.12097814799554978No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTTTTTTTTGTTGGG244210.12034245833805789No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTTTTTTTGTTTGGG243060.11977575825579767No Hit
CGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCC241480.11899716162104017No Hit
CGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCCACCACCAAGACCTAC234080.11535056978736576No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTTTTTTTGTTGGGG232450.11454733401859694No Hit
GGAACGGCTACCGAGGCTCCAGCTTAACGGTATTTGGAGGTCAGCACGGT226390.11156107097642573No Hit
CGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCCTGT224460.11061000040358901No Hit
GTCGAAGTGCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCC206290.10165613910387765No Hit
GGATGTTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTCGAC205530.10128162426690569No Hit
GTCAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGTGCAC205200.10111900598243101No Hit
CGCACGTGGACGACATGCCCAACGCGCTGTCCGCCCTGAGCGACCTGCAC204880.10096131552475862No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGACT190800.056.6591262
AGACTCA270000.047.5313452
CAGACTC281000.046.0931661
GACTCAG297650.043.2335973
ACTCAGA360400.040.1517684
CTCAGAG500100.034.7934071
TCAGAGA511000.033.5993922
CACAGAC348500.032.3350331
AGAACCC532750.031.8027118
AGAGAAC539450.031.568996
GAGAACC544950.031.1838597
CAGAGAG551150.030.911993
GAACCCA562350.030.3607969
GAGAGAA623800.027.5210655
AGAGAGA639500.027.0608084
ACGGACT33000.027.022169
TCGCACA34150.026.5597481
GGTTTTT2228700.026.14720730-34
GGGTTTT2184450.025.26635730-34
TTGGGGG2715100.023.4177345-49
Produced by FastQC (version 0.11.5)