FastQCFastQC Report
Tue 4 Aug 2020
HKMLKDRXX_l01_n02_A1_10___208.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLKDRXX_l01_n02_A1_10___208.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23159398
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTC1188520.5131912323455039No Hit
CAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAA1025130.4426410392878088No Hit
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC871950.37649942368968314No Hit
AGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGC600970.25949292809769925No Hit
CCAAGACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTT462630.19975907836637202No Hit
GCAGAATCCAGATGCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGT345940.14937348544206547No Hit
GTCAACTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCA337040.14553055308259738No Hit
CGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGGCGCACGTGGACG320390.13834124703932288No Hit
CTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTCA319480.1379483180003211No Hit
CGGCTACCGAGGCTCCAGCTTAACGGTATTTGGAGGTCAGCACGGTGCTC271750.11733897400960076No Hit
GTCAAGGCCGCCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGC263190.11364285030206744No Hit
CGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCC262270.11324560336153815No Hit
CGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCCACCACCAAGACCTAC258050.11142344891693645No Hit
CGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCCTGT244440.10554678493801956No Hit
AAGAAAGCGAGCTTAGTGATACTTGTGGGCCAGGGCATTAGCCACACCAG240640.10390598235757251No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGACT285750.051.255392
AGACTCA395300.041.031142
CAGACTC416250.039.4489021
GACTCAG430400.037.7483563
ACTCAGA502550.036.959694
CTCAGAG691300.028.3716181
TCAGAGA682700.028.19742
AGAACCC674650.027.9505468
GAGAACC681150.027.6306047
AGAGAAC698150.027.1447166
GAACCCA726200.026.2559649
CAACTGT196750.025.8528181
GAGAGAA752650.025.3717925
CAGAGAG759050.025.157873
GAATCCA279650.023.8247954
AGAGAGA818450.023.7394754
AGAATCC279450.022.830063
CACAGAC683650.022.5330921
GCAGAAT297350.022.3284071
CACAACT174100.021.630831
Produced by FastQC (version 0.11.5)