FastQCFastQC Report
Tue 4 Aug 2020
HKMLKDRXX_l01_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLKDRXX_l01_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16418019
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTC307820.18748912399236475No Hit
CAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAA240420.1464366681510114No Hit
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC207960.12666570796391455No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC1158200.074.866038
GAGCACA1288000.067.366289
CGGAAGA1265000.067.2918554
TCGGAAG1245400.067.205213
ATCGGAA1284700.066.489612
GATCGGA1260850.066.3012161
AAGAGCA1350400.065.572787
GAAGAGC1419400.060.5430876
GGAAGAG1438450.059.142475
ACAGACT97750.044.538922
CAGACTC121750.036.9566731
CTCAGAG166400.034.1054341
CGGAACA42750.032.3594784
GATCGTA18400.032.3419461
TCAGAGA176000.032.3227732
CAGAGAG181850.031.0660173
GAGAGAA190250.030.303495
CGGAATA26600.029.4067084
ATCGTAA36100.027.1351222
TGTGTTC45500.025.94665
Produced by FastQC (version 0.11.5)