FastQCFastQC Report
Tue 4 Aug 2020
HKMLKDRXX_l01_n01_A1_74___262.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLKDRXX_l01_n01_A1_74___262.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13312206
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC8695096.53166725334629TruSeq Adapter, Index 5 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCGCGTATGC666310.5005256078519218TruSeq Adapter, Index 5 (98% over 50bp)
CTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTC396360.29774178674819185No Hit
CAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAA347380.2609484859233699No Hit
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC318830.2395020028986931No Hit
AGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGC200190.15038078587425704No Hit
CCAAGACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTT154360.11595373448998611No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA2314800.064.4536069
AGAGCAC2352200.063.462698
AAGAGCA2450000.061.0358967
CGGAAGA2501600.059.768674
TCGGAAG2516050.059.2066423
GAAGAGC2540050.058.9405486
ATCGGAA2542150.058.623062
GATCGGA2575800.057.8318981
GGAAGAG2681050.055.8736575
ACAGACT140850.034.923752
AGACTCA164050.030.5597572
ACTCAGA211200.027.7709666
CAGACTC189200.027.0012871
GACTCAG193900.026.0986045
TATGCCG1130950.025.56090745-49
TCGTATG1044250.025.46009340-44
AAGGGGG1084700.025.41562865-69
ATGCCGT1142800.025.3250445-49
AGGGGGG1109950.025.23980965-69
CTCGTAT1058900.025.13112840-44
Produced by FastQC (version 0.11.5)