FastQCFastQC Report
Tue 4 Aug 2020
HKMLKDRXX_l01_n01_A1_73___422.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLKDRXX_l01_n01_A1_73___422.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13121570
Sequences flagged as poor quality0
Sequence length151
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC5965664.546452901596379TruSeq Adapter, Index 4 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGGATGC1154180.8796051082301889TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTTTGC792500.6039673606130973TruSeq Adapter, Index 4 (98% over 50bp)
CTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTC776630.5918727713223341No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATTC689440.5254249300960175TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGG612200.46656002292408605TruSeq Adapter, Index 4 (97% over 49bp)
CAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAA591510.4507920927145151No Hit
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC506560.3860513642803415No Hit
AGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGC412400.3142916586963298No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCGCGTATGC389670.2969690364796286TruSeq Adapter, Index 4 (98% over 50bp)
CCAAGACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTT273870.20871740195723532No Hit
GTCAACTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCA243950.18591525251932503No Hit
CGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGGCGCACGTGGACG232240.17699101555682742No Hit
CGGCTACCGAGGCTCCAGCTTAACGGTATTTGGAGGTCAGCACGGTGCTC226520.17263178110546223No Hit
GCAGAATCCAGATGCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGT221810.1690422716184115No Hit
CGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCC213070.1623814833133535No Hit
CTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTCA208330.15876911070855088No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGGTTGC205750.15680288258188615TruSeq Adapter, Index 4 (97% over 45bp)
GGAACGGCTACCGAGGCTCCAGCTTAACGGTATTTGGAGGTCAGCACGGT189150.14415195742582634No Hit
CGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCCTGT187590.14296307530272673No Hit
CGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCCACCACCAAGACCTAC185680.14150745680585478No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGGATGG180390.13747592704226705TruSeq Adapter, Index 4 (97% over 49bp)
GTCAAGGCCGCCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGC179020.13643184466492958No Hit
CAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTC163990.12497742267121997No Hit
CGGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGC161690.12322458364357314No Hit
GTCAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGTGCAC161530.12310264701556291No Hit
GCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGACCGGGTC157230.1198256001377884No Hit
GTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCACCA156370.11917019076223348No Hit
GAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGG153030.11662476365252024No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTGTGC147860.11268468635994015TruSeq Adapter, Index 4 (98% over 50bp)
GGATGTTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTCGAC146920.11196830867038014No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCGCGGATGC146880.1119378245133776TruSeq Adapter, Index 4 (97% over 45bp)
CAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAA146860.11192258243487632No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTTTGG145200.11065748991927032TruSeq Adapter, Index 4 (97% over 49bp)
GCCGGTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGA143940.10969723897368988No Hit
GTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTCCTGAGGA137880.10507888918780298No Hit
CGCACGTGGACGACATGCCCAACGCGCTGTCCGCCCTGAGCGACCTGCAC137760.10498743671679533No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA2433700.076.242049
AGAGCAC2470800.075.176468
AAGAGCA2548450.072.9911357
CGGAAGA2623150.071.013144
GAAGAGC2629300.070.79086
TCGGAAG2641550.070.3104253
ATCGGAA2668500.069.753622
GATCGGA2696500.068.9824751
GGAAGAG2741700.067.937275
ACAGACT139200.053.8401262
AGACTCA199150.045.538082
CAGACTC213700.042.479131
GACTCAG223600.041.0174833
ACTCAGA264250.039.3716774
TCAGAGA350450.031.7780532
CTCAGAG366000.030.7109451
AGAACCC376800.029.381138
AGAGAAC383350.029.0682436
CAACTGT83950.028.7836841
GAGAACC387450.028.5922327
Produced by FastQC (version 0.11.5)