FastQCFastQC Report
Tue 4 Aug 2020
HKMLKDRXX_l01_n01_A1_6___148.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLKDRXX_l01_n01_A1_6___148.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27961691
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC13841524.950172720240704TruSeq Adapter, Index 11 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCGCGTATGC1172400.4192879464979425TruSeq Adapter, Index 11 (98% over 50bp)
CTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTC1089690.38970819039520893No Hit
CAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAA728620.2605779457329673No Hit
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC634320.22685323287493592No Hit
GCAGAATCCAGATGCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGT559690.20016314463957133No Hit
AGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGC547590.19583579548175398No Hit
CCAAGACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTT411360.14711556607931903No Hit
GTCAACTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCA371180.1327459058180709No Hit
AAGAAAGCGAGCTTAGTGATACTTGTGGGCCAGGGCATTAGCCACACCAG350830.12546809132537798No Hit
CTCAAGGGCACCTTTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCA330870.11832975337578833No Hit
GCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGG327050.11696359851770051No Hit
CTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTCA323400.11565824112711924No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCAACACTAAATCCCTAAACC318870.11403816743415125TruSeq Adapter, Index 7 (97% over 35bp)
CGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGGCGCACGTGGACG287700.10289077295074893No Hit
GTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCC283640.10143878637382839No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGACT259800.038.0895162
AGACTCA335600.034.8476372
CAGACTC347250.033.915741
GAGCACA7705750.033.7343989
AGAGCAC7841400.033.1711588
AAGAGCA8083750.032.2197387
GACTCAG366950.032.2050743
CAACTGT250850.032.1384541
GAAGAGC8318100.031.2980546
CGGAAGA8374300.031.0562554
ACTCAGA439850.030.856264
TCGGAAG8464300.030.6267873
ATCGGAA8586850.030.2332782
GGAAGAG8629500.030.1789975
GATCGGA8655300.029.9353351
AGAACCC572950.029.4454218
GAGAACC589650.028.53777
AGAGAAC612250.027.804016
CTCAGAG646550.027.542131
GAACCCA622450.027.3949819
Produced by FastQC (version 0.11.5)