FastQCFastQC Report
Tue 4 Aug 2020
HKMLKDRXX_l01_n01_A1_5___146.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLKDRXX_l01_n01_A1_5___146.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23823145
Sequences flagged as poor quality0
Sequence length151
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC756121531.738945466687962TruSeq Adapter, Index 10 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGTATGC6826212.8653689510767784TruSeq Adapter, Index 10 (98% over 50bp)
CTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTC1053950.442405904006377No Hit
CAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAA780560.3276477559952727No Hit
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC744310.31243146108542763No Hit
AGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGC489100.2053045473215228No Hit
CCAAGACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTT405630.17026719184221897No Hit
GTCAACTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCA364880.15316197756425526No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCAAAACAATATCCCTAAACC363710.15267085852854442TruSeq Adapter, Index 1 (97% over 34bp)
GCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGG358050.15029501772331066No Hit
CTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTCA325030.1364345471599153No Hit
CGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGGCGCACGTGGACG281670.11823375964844272No Hit
CGGCTACCGAGGCTCCAGCTTAACGGTATTTGGAGGTCAGCACGGTGCTC251160.10542688633259799No Hit
GCAGAATCCAGATGCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGT247580.10392414603529468No Hit
CGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCC241830.10151052684269855No Hit
GTCAAGGCCGCCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGC239620.10058285755302249No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA12744400.0101.771279
AGAGCAC12830750.0101.1694268
AAGAGCA12913400.0100.6377
CGGAAGA13070350.099.574084
GAAGAGC13062600.099.518066
TCGGAAG13109950.099.0519563
ATCGGAA13202300.098.607852
GGAAGAG13215750.098.430295
GATCGGA13264000.098.354671
GAACGGA68000.066.160511
ACAGACT179250.063.988932
AACGGAA85650.055.311822
TCGGAAA61050.047.620963
ACGGAAG98150.046.3883323
ATTCGGA24800.046.2208062
AGACTCA255750.045.413454
ATCGGAG101950.043.550822
CAGACTC269200.043.117713
GACTCAG277300.041.7011875
ACTCAGA337650.038.9071164
Produced by FastQC (version 0.11.5)