FastQCFastQC Report
Tue 4 Aug 2020
HKMLKDRXX_l01_n01_A1_4___135.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLKDRXX_l01_n01_A1_4___135.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20292921
Sequences flagged as poor quality0
Sequence length151
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC397336719.580064397826217TruSeq Adapter, Index 9 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCGCGTATGC3098901.5270842477531943TruSeq Adapter, Index 9 (98% over 50bp)
CTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTC1135760.5596828568937907No Hit
CAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAA784700.38668656917355565No Hit
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC705380.34759904697800775No Hit
AGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGC556040.27400688151301633No Hit
CCAAGACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTT417380.2056776350728414No Hit
GTCAACTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCA397770.1960141667136042No Hit
GCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGG361980.17837747458830594No Hit
CTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTCA332610.16390444727006034No Hit
CGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGGCGCACGTGGACG307700.1516292307056239No Hit
CGGCTACCGAGGCTCCAGCTTAACGGTATTTGGAGGTCAGCACGGTGCTC305800.15069294361319396No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCAACAAAATATCCCTAAACC303840.14972708955995048TruSeq Adapter, Index 7 (97% over 35bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT296490.1461051368602874No Hit
GGAACGGCTACCGAGGCTCCAGCTTAACGGTATTTGGAGGTCAGCACGGT256410.1263544070368184No Hit
CGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCC251740.12405311192016172No Hit
GTCAAGGCCGCCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGC248500.1224564960362286No Hit
CGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCCACCACCAAGACCTAC238050.11730691702786404No Hit
CGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCCTGT226250.11149208140119404No Hit
GTCGAAGTGCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCC224600.1106789899788207No Hit
GTCAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGTGCAC222320.1095554454679048No Hit
CGCACGTGGACGACATGCCCAACGCGCTGTCCGCCCTGAGCGACCTGCAC215010.10595320407545074No Hit
CTCAGGTCGAAGTGCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAA204240.10064593460941379No Hit
CTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCACCTCC203730.1003946154424984No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA8727250.080.525589
AGAGCAC8813500.079.801718
AAGAGCA8958400.078.632347
GAAGAGC9122450.077.227026
CGGAAGA9163750.076.9657754
TCGGAAG9206350.076.420643
ATCGGAA9290450.075.891242
GGAAGAG9311450.075.726245
GATCGGA9326400.075.5976261
ACAGACT186750.059.205562
AGACTCA264500.045.943842
CAGACTC280900.043.296471
GACTCAG296000.041.4181143
GAACGGA59250.041.02861
ACTCAGA356500.038.9853064
CTCAGAG493850.035.368061
TCAGAGA498950.034.5047452
CACAGAC348550.033.165071
AGAACCC517400.032.844788
GAGAGAA532650.032.3402185
Produced by FastQC (version 0.11.5)