FastQCFastQC Report
Tue 4 Aug 2020
HKMLKDRXX_l01_n01_A1_10___208.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLKDRXX_l01_n01_A1_10___208.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23159398
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTC1229570.5309162181158595No Hit
CAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAA1035890.4472871013313904No Hit
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC883950.38168090552267375No Hit
AGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGC623900.2693938763002389No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGGTT602730.2602528787665379TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGGGT470250.2030493193303211TruSeq Adapter, Index 15 (97% over 40bp)
CCAAGACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTT458500.19797578503551777No Hit
GCAGAATCCAGATGCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGT359660.15529764633778478No Hit
CTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTCA356810.1540670444024495No Hit
GTCAACTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCA339290.14650208092628314No Hit
CGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGGCGCACGTGGACG332060.14338023812190626No Hit
CGGCTACCGAGGCTCCAGCTTAACGGTATTTGGAGGTCAGCACGGTGCTC309260.13353542263922405No Hit
CGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCC286280.12361288492904696No Hit
GTCAAGGCCGCCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGC281770.12166551134014797No Hit
GGAACGGCTACCGAGGCTCCAGCTTAACGGTATTTGGAGGTCAGCACGGT273530.11810756048149437No Hit
CGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCCACCACCAAGACCTAC270990.11701081349351136No Hit
GTCAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGTGCAC258100.11144503842457391No Hit
CGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCCTGT257000.11097006925654974No Hit
AAGAAAGCGAGCTTAGTGATACTTGTGGGCCAGGGCATTAGCCACACCAG243310.10505886206541293No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGACT269500.049.126692
AGACTCA367300.041.9119532
CAGACTC390900.039.686581
GACTCAG408500.037.9272273
ACTCAGA476050.036.611754
TCAGAGA652150.028.9783522
AGAACCC644800.028.8739228
CTCAGAG668000.028.839631
GAGAACC658800.028.172297
AGAGAAC668450.027.8957376
GAACCCA698600.026.98249
GAGAGAA718750.026.2765585
CAGAGAG731450.025.8500613
CAACTGT195400.025.251431
AGAGAGA802450.023.9694394
GAATCCA287750.023.0790944
CACAACT169650.022.1125241
AGAATCC284400.021.9998553
ACAACTG170350.021.1654592
CACTAGC200750.020.8020214
Produced by FastQC (version 0.11.5)