FastQCFastQC Report
Wed 1 Jul 2020
HKMKFDRXX_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMKFDRXX_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60045963
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGATCTCGTATGCCGTCTTCTGCTTGAAAAAGGGGGGGGGGGGGGGGG1045210.17406832162888286RNA PCR Primer, Index 23 (100% over 28bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC2681400.044.9852148
CGGAAGA2939400.043.555494
TCGGAAG3011400.042.870483
ATCGGAA3113450.042.0921482
GAGCACA2887750.041.483459
GATCGGA3191200.041.279821
GAAGAGC3192300.038.843416
AAGAGCA3171300.038.762467
GTGGATC764800.036.876191
GGAAGAG3478500.036.2682885
TGGATCT751000.033.9546552
GGATCTC753900.033.774483
ACGTAAG102050.029.40042532-33
CGTAAGA102600.029.2428232-33
TCACGTA285100.028.81538630-31
CGTAGGA73300.027.62260232-33
CGTCTGA2094500.027.26122916-17
CACGTCT2138950.026.93471114-15
GAACTCC2044600.026.82418420-21
ACGTAGG74500.026.63603432-33
Produced by FastQC (version 0.11.5)