FastQCFastQC Report
Wed 1 Jul 2020
HKMKFDRXX_n01_mb302.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMKFDRXX_n01_mb302.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45000154
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGC3852600.8561304034648415TruSeq Adapter, Index 16 (98% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT590350.036.20290450-51
CGTCTTC586200.035.83925650-51
TGCCGTC608350.035.3074848-49
GCCGTCT598200.035.22345748-49
ATGCCGT624650.033.7471846-47
TATGCCG640250.033.52975546-47
TCGTATG590850.032.37182242-43
CGTATGC667000.032.1244644-45
CTCGTAT617450.031.38123142-43
AAGGGGG689950.030.60967866-67
AGGGGGG618400.029.9654166-67
GCTTGAA701900.029.96113858-59
TCTCGTA645100.029.68969340-41
GTATGCC729100.029.02661744-45
ATCTCGT676000.028.6735140-41
TGCTTGA749450.028.49750758-59
CATCTCG692950.027.60868538-39
CTGCTTG812600.025.8788356-57
AAAAGGG855450.024.53423964-65
GTCTTCT911350.023.54042452-53
Produced by FastQC (version 0.11.5)