FastQCFastQC Report
Wed 1 Jul 2020
HKMKFDRXX_n01_mb301.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMKFDRXX_n01_mb301.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52615565
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTCGTATGC5772511.0971107123909056TruSeq Adapter, Index 15 (98% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT824550.038.27812650-51
GCCGTCT823600.038.14628248-49
CGTCTTC823750.038.04137450-51
TGCCGTC844350.037.66465448-49
ATGCCGT862750.036.69339846-47
TATGCCG887800.035.877546-47
TCGTATG834300.034.99388542-43
CGTATGC914000.034.8230744-45
CTCGTAT861600.034.06983642-43
TCTCGTA891700.032.8140740-41
AAGGGGG971600.032.62793766-67
GCTTGAA961750.032.24020858-59
GTATGCC989100.032.15501844-45
ATCTCGT932600.031.54822740-41
AATCTCG937150.031.09588838-39
AGGGGGG882500.030.84154966-67
TGCTTGA1012100.030.31249658-59
CTGCTTG1090300.028.10468156-57
AAAAGGG1153100.027.43294564-65
GTCTTCT1198400.026.02243652-53
Produced by FastQC (version 0.11.5)