FastQCFastQC Report
Wed 1 Jul 2020
HKMKFDRXX_n01_mb300.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMKFDRXX_n01_mb300.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49637529
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGC41593028.379349423296233TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCGCGTATGC3335650.6720016219985487TruSeq Adapter, Index 22 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA6621750.070.005329
AGAGCAC6691600.069.425758
CGGAAGA6813700.069.2243044
TCGGAAG6905500.068.591443
GAAGAGC6829200.068.319346
ATCGGAA7041400.067.617722
GATCGGA7116200.066.967141
AAGAGCA7008050.066.4800347
GGAAGAG7179250.065.275375
CTCGTAT4648500.045.49963442-43
CCGTCTT4959250.045.3941950-51
CGTATGC5043100.045.36804244-45
TATGCCG5045950.045.3598446-47
TGCCGTC5036250.045.3391648-49
ATCTCGT4699650.045.2431740-41
TCGTATG4615600.044.55158242-43
TCTCGTA4659900.044.4395740-41
ACGGTAG5278150.044.37709832-33
GCCGTCT4954400.044.16049648-49
CGTCTTC4940200.044.15232550-51
Produced by FastQC (version 0.11.5)