FastQCFastQC Report
Wed 1 Jul 2020
HKMKFDRXX_n01_mb299.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMKFDRXX_n01_mb299.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63028392
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTCGTATGC1093715117.35273684278666TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCGCGTATGC9773171.5505980225546607TruSeq Adapter, Index 7 (97% over 36bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA17145250.071.3162169
AGAGCAC17286250.070.877378
GAAGAGC17688400.069.815076
CGGAAGA17932250.069.435894
AAGAGCA17742050.069.367467
TCGGAAG18138900.068.906833
GGAAGAG18174450.068.203125
ATCGGAA18418550.068.084482
GATCGGA18598600.067.5249561
CTCGTAT12219850.045.57352442-43
CCGTCTT13280000.045.54661650-51
TGCCGTC13398600.045.5204148-49
TATGCCG13429000.045.4742946-47
CGTATGC13453850.045.38217544-45
ATCTCGT12297000.045.35605240-41
AAGGGGG13337650.045.04629566-67
GGATCTC12449900.044.89789638-39
TGCTTGA13058350.044.81234458-59
TCGTATG12177500.044.52556242-43
GTCTTCT13315550.044.40482352-53
Produced by FastQC (version 0.11.5)