FastQCFastQC Report
Wed 1 Jul 2020
HKMKFDRXX_n01_mb298.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMKFDRXX_n01_mb298.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52667140
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATGC4477230.8500993218921704TruSeq Adapter, Index 22 (98% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT655350.036.8195150-51
GCCGTCT655500.036.19119648-49
TGCCGTC675400.036.13435448-49
CGTCTTC656150.036.12665650-51
ATGCCGT689200.034.8179846-47
TATGCCG705800.034.63860746-47
CGTATGC727150.033.6084944-45
TCGTATG667350.033.6044742-43
CTCGTAT693300.032.98057642-43
TCTCGTA719300.031.35912140-41
ATCTCGT752600.030.51457440-41
AAGGGGG811550.030.31644466-67
GATCTCG751400.030.17430138-39
GTATGCC807900.029.91114444-45
CGATCTC771150.029.8728938-39
GCTTGAA786300.029.61770458-59
AGGGGGG730000.028.1011566-67
ACGATCT807450.028.04144136-37
TGCTTGA829250.027.7772558-59
TACGATC925400.026.83960336-37
Produced by FastQC (version 0.11.5)