FastQCFastQC Report
Wed 1 Jul 2020
HKMKFDRXX_n01_mb297.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMKFDRXX_n01_mb297.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45747647
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATCTCGTATGC4372420.9557693754172756TruSeq Adapter, Index 21 (98% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT686150.034.935150-51
CGTCTTC684500.034.3010250-51
TGCCGTC704550.034.21835348-49
GCCGTCT691500.034.00866748-49
ATGCCGT719950.032.8297746-47
TATGCCG742300.032.5165946-47
CGTATGC769450.031.32598344-45
TCGTATG696400.031.20683942-43
CTCGTAT719800.030.78641542-43
AAGGGGG780200.030.43877666-67
GCTTGAA791650.029.66636858-59
AGGGGGG698400.029.23316266-67
TCTCGTA747950.029.1067940-41
GTATGCC836150.028.4122844-45
ATCTCGT785150.028.25425740-41
TGCTTGA843100.028.16583858-59
GATCTCG788800.027.5993338-39
CGATCTC810350.027.28756738-39
TCGATCT845100.025.64260336-37
CTGCTTG916500.025.4741856-57
Produced by FastQC (version 0.11.5)