FastQCFastQC Report
Wed 1 Jul 2020
HKMKFDRXX_n01_mb296.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMKFDRXX_n01_mb296.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47966779
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTCGTATGC556246611.596496817099185TruSeq Adapter, Index 20 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCGCGTATGC4735400.987224929153571TruSeq Adapter, Index 20 (97% over 42bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA9510400.064.036089
AGAGCAC9631050.063.353738
GAAGAGC9917250.061.8599246
CGGAAGA9986300.061.8036354
AAGAGCA9999450.061.294987
TCGGAAG10121000.061.1417963
ATCGGAA10262450.060.3776972
GATCGGA10376050.059.7474021
GGAAGAG10296100.059.732685
CCGTCTT6671450.045.53505750-51
TGCCGTC6714950.045.43989648-49
TATGCCG6736400.045.3298446-47
CTCGTAT6168050.045.3131742-43
CGTATGC6754550.045.17218844-45
ATCTCGT6213500.044.94537740-41
AAGGGGG6671700.044.58112766-67
TCGTATG6149050.044.5808242-43
CGTCTTC6672350.044.50120550-51
GCCGTCT6691200.044.48278448-49
TGCTTGA6764250.044.39564558-59
Produced by FastQC (version 0.11.5)