FastQCFastQC Report
Wed 1 Jul 2020
HKMKFDRXX_n01_mb295.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMKFDRXX_n01_mb295.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54716736
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCTCGTATGC54066519.88116506072292TruSeq Adapter, Index 19 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCGCGTATGC3806530.6956792890570082TruSeq Adapter, Index 19 (97% over 42bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA10172300.058.5868039
AGAGCAC10328050.057.857818
GAAGAGC10604250.056.5380746
CGGAAGA10687600.056.4104964
AAGAGCA10712900.055.90757
TCGGAAG10862950.055.651613
ATCGGAA11053950.054.8223462
GGAAGAG11052650.054.4164735
GATCGGA11215000.054.1083341
CCGTCTT6518550.044.63908850-51
CGTCTTC6499400.044.6050750-51
TGCCGTC6580800.044.5403548-49
GCCGTCT6535500.044.5099248-49
ATGCCGT6602200.044.32871246-47
TATGCCG6625300.044.30826646-47
TCGTATG6159500.044.23017542-43
CTCGTAT6194250.044.1431242-43
CGTATGC6653950.044.08786844-45
AAGGGGG6569000.044.04737566-67
TCTCGTA6233250.043.7890340-41
Produced by FastQC (version 0.11.5)