FastQCFastQC Report
Wed 1 Jul 2020
HKMKFDRXX_n01_mb294.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMKFDRXX_n01_mb294.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55693035
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT285150.09.054378550-51
ATGCCGT290750.08.70195446-47
CGTCTTC301150.08.57332250-51
TCGTATG273100.08.44651642-43
TGCCGTC310150.08.41807148-49
TATGCCG308650.08.405107546-47
GCCGTCT309050.08.27891948-49
CTCGTAT292050.07.98793242-43
CGTATGC334250.07.676075544-45
TCTCGTA316400.07.284036640-41
GCTTGAA379200.07.14600758-59
ATCTCGT358150.06.62067740-41
AGGGGGG403350.06.34111966-67
GTATGCC413350.06.281872744-45
TGCTTGA444850.06.251574558-59
AAGGGGG477750.06.148954466-67
CGCATCG155050.06.14182594-95
TCGCGTA97100.06.016455794-95
CGCGTAT89300.05.98350994-95
ATCGCGT109500.05.725516394-95
Produced by FastQC (version 0.11.5)