FastQCFastQC Report
Wed 1 Jul 2020
HKMKFDRXX_n01_mb293.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMKFDRXX_n01_mb293.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57723611
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATGC56917019.860264978918245TruSeq Adapter, Index 3 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGAGATCTCGTATGC21747673.7675518948390114TruSeq Adapter, Index 20 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCGCGTATGC4422290.7661145800459365TruSeq Adapter, Index 3 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGAGATCGCGTATGC1185380.20535444326239397TruSeq Adapter, Index 20 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA10461950.084.066532
GATCGGA10485900.083.924931
TCGGAAG10460000.083.924863
CGGAAGA10520100.083.192774
GAGCACA10568450.081.641319
AGAGCAC10609300.081.4688648
GAAGAGC10721850.081.050776
AAGAGCA10866400.079.8353047
GGAAGAG11155650.078.130255
CTCGTAT8654250.045.7186942-43
CGTATGC9325800.045.5120544-45
TATGCCG9430150.045.44172746-47
ATCTCGT8726600.045.4379540-41
CCGTCTT9309050.045.4346150-51
TGCCGTC9424650.045.36941548-49
TCGTATG8622700.044.94399642-43
TCTCGTA8668850.044.83235540-41
TGCTTGA9070150.044.70674558-59
ACGTAGA7116250.044.5989732-33
GATCTCG8776450.044.49727238-39
Produced by FastQC (version 0.11.5)