FastQCFastQC Report
Wed 1 Jul 2020
HKMKFDRXX_n01_mb292.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMKFDRXX_n01_mb292.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46069273
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC608180.13201423864448653TruSeq Adapter, Index 11 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT297350.012.10179250-51
CGTCTTC304300.011.77075750-51
ATGCCGT311400.011.32162346-47
TGCCGTC319550.011.27820348-49
TCGTATG283350.011.25159242-43
GCCGTCT317000.011.15159148-49
TATGCCG326550.010.9127746-47
CTCGTAT303650.010.63238942-43
CGTATGC350450.010.1685444-45
TCTCGTA324850.09.87372640-41
GCTTGAA385950.09.48855558-59
ATCTCGT360850.09.02694740-41
AGGGGGG375950.08.81201766-67
AAGGGGG442600.08.74595866-67
TGCTTGA433100.08.69684558-59
GTATGCC420850.08.50705644-45
CATCTCG400200.07.89592238-39
AAAAGGG521800.07.464483364-65
AAAGGGG523100.06.487949464-65
CTGCTTG625700.06.171490756-57
Produced by FastQC (version 0.11.5)