FastQCFastQC Report
Wed 1 Jul 2020
HKMKFDRXX_n01_mb291.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMKFDRXX_n01_mb291.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50245395
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC26260275.226403335071801TruSeq Adapter, Index 10 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGTATGC2280880.45394806827570966TruSeq Adapter, Index 10 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA4027850.072.166754
TCGGAAG4063050.071.69653
ATCGGAA4082350.071.557772
GATCGGA4128100.070.809431
GAGCACA4100950.070.282129
AGAGCAC4145400.069.615588
GAAGAGC4251850.068.116726
AAGAGCA4382950.066.045347
GGAAGAG4661900.062.310775
CTCGTAT2916950.045.08429742-43
CGTATGC3206700.044.8153944-45
TATGCCG3211950.044.7791746-47
TCGTATG2910700.044.75913242-43
TCTCGTA2921650.044.58795540-41
TGCCGTC3229250.044.52897348-49
CCGTCTT3216700.044.4940850-51
ATCTCGT2956250.044.48803740-41
ATGCCGT3205300.044.402946-47
TATCTCG2941200.044.261638-39
GCCGTCT3224900.044.0615748-49
Produced by FastQC (version 0.11.5)