FastQCFastQC Report
Wed 1 Jul 2020
HKMKFDRXX_n01_mb290.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMKFDRXX_n01_mb290.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55081192
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC10568231.9186639969592523TruSeq Adapter, Index 9 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCGCGTATGC816690.1482702117267179TruSeq Adapter, Index 9 (98% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT1649950.036.56340850-51
TGCCGTC1710500.035.50093548-49
TATGCCG1738850.034.93992246-47
AAGGGGG1728150.034.5295366-67
TGCTTGA1710550.034.5225858-59
CGTCTTC1652200.034.4449150-51
GCTTGAA1670150.033.9171958-59
CTCGTAT1644650.033.7844842-43
GCCGTCT1690600.033.7730548-49
CGTATGC1797200.033.7460444-45
ATGCCGT1708100.033.5633646-47
AAAAGGG1776600.032.8672864-65
TCGTATG1610500.032.84729442-43
ATCTCGT1747500.031.81846240-41
ATCGGGA132200.031.3677272
TCTCGTA1686250.031.36375440-41
GATCGGG134600.031.306011
GTATGCC1860300.030.87484744-45
CTTGAAA1986400.030.31525860-61
AGGGGGG1670900.030.29183266-67
Produced by FastQC (version 0.11.5)